RNA-seq in Colias erate. Colias erate

A transcriptome data obtained by RNA sequence (RNA-seq) from a mixture of total-RNA extracted from one male and one female pupae in eastern pale clouded yellow butterfly, Colias erate. We acquired 100-base-paired reads using Hiseq 2500 (Illumina) and conducted de novo assemble to produce contig data by Trinity. The sequence information was further used to design single tagged site (STS) primer pairs and then selected the bacterial artificial chromosome (BAC) clones containing Bombyx mori orthologs. We extracted the BAC-DNA to make probes for BAC-FISH mapping in C. erate.本数据集源自对橙黄豆粉蝶（Colias erate）1头雄蛹与1头雌蛹的混合总RNA进行RNA测序（RNA-seq）所获得的转录组数据。研究团队采用Illumina HiSeq 2500测序平台获取了100 bp双端测序读段，通过Trinity软件进行从头组装以得到重叠群（contig）数据。随后利用该序列信息设计单标记位点（STS）引物对，并筛选出携带家蚕（Bombyx mori）同源基因的细菌人工染色体（BAC）克隆。最后提取该BAC的DNA以制备探针，用于橙黄豆粉蝶（C. erate）的BAC荧光原位杂交（FISH）定位分析。