Gene expression of sorghum-specific Exserohilum turcicum strain

Exserohilum turcicum is a fungal pathogen that causes northern corn leaf blight and sorghum leaf blight. However, strains are host-specific, meaning a strain typically only causes disease on either maize or sorghum. This pathosystem provides a unique opportunity to identify the mechanisms underlying host specificity through genomic and transcriptomic studies. To characterize host specificity, we (i) constructed de novo annotated assemblies of one sorghum- (15St008) and one maize-specific (Et52B) E. turcicum strain, ii) identified and characterized structural changes between the two genomes, and (iii) compared differential gene expression. This GEO project contains the gene expression of the sorghum-specific strain (15St008) during in planta infection of sorghum and in axenic cultures. RNA-seq profiling of strain 15St008 during in planta infection and in axenic cultures. In planta samples were collected 10 days after inoculations and mycelium from axenic liquid cultures were collected after 5 days of growth.大斑突脐蠕孢菌（Exserohilum turcicum）是一类可引发玉米北方叶枯病与高粱叶枯病的真菌病原菌。该病原菌的菌株具有宿主特异性，即单株菌株通常仅能在玉米或高粱其中一种宿主上引发病害。该寄主-病原病害系统为通过基因组学与转录组学研究解析宿主特异性的潜在分子机制提供了独特的研究契机。为表征宿主特异性，本研究团队开展了以下三项工作：（1）构建了1株高粱专化型菌株（15St008）与1株玉米专化型菌株（Et52B）的从头注释基因组组装序列；（2）鉴定并分析了两个基因组之间的结构变异；（3）比较了二者的差异基因表达情况。本GEO项目包含了高粱专化型菌株15St008在植物体内侵染高粱以及无菌纯培养条件下的基因表达数据。本研究对菌株15St008在植物体内侵染与无菌纯培养状态下的样本进行了RNA测序（RNA-seq）分析：植物体内侵染样本于接种后10天采集，无菌液体培养基中的菌丝体则于培养5天后采集。