Nanoscale modifications in the early heating stages of bone are heterogeneous at the microstructural scale

Nanoscale studies of bone provide key indicators to evidence subtle structural changes that may occur in the biomedical, forensic and archaeological contexts. One specific problem encountered in all those disciplines, for which the identification of nanostructural cues could prove useful, is to properly monitor the effect of heating on bone tissue. In particular, the mechanisms at work at the onset of heating are still relatively unclear. Using a multiscale approach combining Raman microspectroscopy, transmission electron microscopy (TEM), synchrotron quantitative scanning small-angle X-ray scattering imaging (qsSAXSI) and polarized light (PL) microscopy, we investigate the ultrastructure of cortical bovine bone heated at temperatures骨的纳米尺度研究可为生物医学、法医及考古场景中可能出现的细微结构变化提供关键指示依据。在上述所有学科领域中，均存在一类可通过识别纳米结构特征予以有效解决的特定问题，即精准监测热作用对骨组织的影响。尤为关键的是，加热初期的作用机制目前仍相对不明晰。本研究采用拉曼显微光谱学（Raman microspectroscopy）、透射电子显微镜（Transmission Electron Microscopy, TEM）、同步辐射定量扫描小角X射线散射成像（synchrotron quantitative scanning small-angle X-ray scattering imaging, qsSAXSI）以及偏振光显微镜（Polarized Light Microscopy, PL）相结合的多尺度研究方法，对经加热处理的牛皮质骨的超微结构展开研究。