KEGG enrichment analysis.

BackgroundMicroplastics (MPs), ubiquitous environmental pollutants, are increasingly associated with global health risks, yet their role in allergic rhinitis (AR) pathogenesis remains poorly understood.MethodsToxicity profiles of four typical MPs (polyethylene [PE], polypropylene [PP], polyvinyl chloride [PVC], polystyrene [PS]) were evaluated using ADMETlab 3.0. MP-related targets and AR-associated genes were integrated from the CTD database and GSE43523 dataset. Functional enrichment (GO/KEGG) and PPI network analysis (STRING/GeneMANIA) were performed on overlapping genes. LASSO regression and expression validation identified key targets, and molecular docking (Autodock Vina) assessed interactions with potential therapeutics predicted by CTD.ResultsADMET analysis revealed MPs exhibit significant respiratory toxicity and ocular toxicity. We identified 301 MP toxicity targets, 1,026 AR differentially expressed genes (DEGs), and 15 overlapping pathogenic targets. Functional enrichment (GO/KEGG) demonstrated MPs disrupt respiratory mucosal homeostasis via apoptosis, mitochondrial autophagy, and inflammatory pathways. PPI network analysis and LASSO regression pinpointed DNAJB9, SQSTM1, and MAPK9 as core mediators: these genes were significantly downregulated in AR patients (P ConclusionsMPs drive AR pathogenesis through respiratory toxicity pathways, with DNAJB9, SQSTM1, and MAPK9 serving as critical molecular mediators. Resveratrol, by modulating target-mediated programmed cell death, emerges as a promising therapeutic candidate for mitigating MP-induced AR.背景 微塑料（Microplastics, MPs）作为广泛分布的环境污染物，其与全球健康风险的关联日益受到重视，但迄今其在变应性鼻炎（Allergic Rhinitis, AR）发病机制中的作用仍未得到充分阐明。 方法 本研究采用ADMETlab 3.0平台，对4种典型微塑料即聚乙烯（Polyethylene, PE）、聚丙烯（Polypropylene, PP）、聚氯乙烯（Polyvinyl Chloride, PVC）及聚苯乙烯（Polystyrene, PS）的毒性谱进行评估。从比较毒理基因组学数据库（Comparative Toxicogenomics Database, CTD）及GSE43523数据集筛选整合微塑料相关靶点与变应性鼻炎关联基因。对重叠基因开展基因本体/京都基因与基因组百科全书（Gene Ontology/Kyoto Encyclopedia of Genes and Genomes, GO/KEGG）功能富集分析及基于STRING、GeneMANIA数据库的蛋白质相互作用（Protein-Protein Interaction, PPI）网络分析。通过LASSO回归与表达验证筛选核心靶点，并采用分子对接工具Autodock Vina评估其与CTD预测的潜在治疗药物的相互作用。 结果 ADMET分析显示，微塑料具有显著的呼吸道毒性与眼毒性。本研究共筛选得到301个微塑料毒性靶点、1026个变应性鼻炎差异表达基因（Differentially Expressed Genes, DEGs）及15个重叠致病靶点。GO/KEGG功能富集分析结果表明，微塑料可通过细胞凋亡、线粒体自噬及炎症通路破坏呼吸道黏膜稳态。蛋白质相互作用网络分析与LASSO回归确定DNAJB9、SQSTM1及MAPK9为核心介导因子：上述基因在变应性鼻炎患者体内呈显著下调（P 结论 微塑料可通过呼吸道毒性通路介导变应性鼻炎的发病，其中DNAJB9、SQSTM1及MAPK9发挥关键分子介导作用。白藜芦醇可通过调控靶点介导的程序性细胞死亡，成为缓解微塑料诱导的变应性鼻炎的潜在候选治疗药物。