K_D determination of DB3 and DB3DB3 to monomeric Aβ using biolayer interferometry (BLI).

N-terminally biotinylated Aβ(1–42) monomers were immobilized on streptavidin biosensors and the binding of DB3 and DB3DB3 was detected. Representative double referenced sensorgrams of a dilution series of DB3 (A) and DB3DB3 (B) are shown, including the equilibrium dissociation constants (KD) as means ± SD of data recorded in triplicate. For steady state analysis Langmuir´s 1:1 binding model was applied. Representative fits of DB3 (C) and DB3DB3 (D) are depicted with the corresponding corrected R2.将N端生物素化的Aβ(1–42)单体固定于链霉亲和素生物传感器表面，用于检测DB3与DB3DB3的结合反应。本文展示了DB3（图A）与DB3DB3（图B）浓度梯度稀释系列的典型双参比传感图，其中平衡解离常数（KD）为三次重复实验所得数据的平均值±标准差。稳态分析采用朗缪尔1:1结合模型，图C（对应DB3）与图D（对应DB3DB3）呈现了对应的代表性拟合曲线，并标注了相应的校正后R²值。