Genomic features shaping the landscape of meiotic double-strand break hotspots in maize [MNase-Seq]. Zea mays

We report the application of next generation sequencing techonology to study nucleosomal occupancy in flowers containing zygotene meiocytes. The results show DSB enrichment in nucleosome depleted regions associated with RAD51 Overall design: To identify distribution characters of nucleosomal occupancy, we compared the genomic distribution of MNase-Seq reads to Illumina-sequenced meiotic input

本研究报道了应用下一代测序技术（next generation sequencing），对携带有合线期减数分裂细胞的花组织中的核小体占位特征展开研究的相关工作。研究结果表明，在与RAD51相关的核小体缺失区域中存在DNA双链断裂（Double-Strand Break, DSB）的富集现象。整体实验设计：为明确核小体占位的分布特征，我们将微球菌核酸酶测序（Micrococcal Nuclease sequencing, MNase-Seq）的读段基因组分布与经Illumina测序的减数分裂输入样本进行了比对分析。