Effects of the Schlechtendalia chinensis extract on Arabidopsis seedlings

An extract of a gall-inducing aphid, Schlechtendalia chinensis, induces excessive or ectopic nutritive, vascular, and protective tissues, as observed in typical insect galls. This analysis is a part of the Arabidopsis-based Gall Formation Assay (Ab-GALFA), and shows its availability as a model system for analyzing the molecular mechanisms of gall formation. This work provided the following conditions. Arabidopsis wild-type Columbia (Col-0) was grown on vertical half-strength Murashige and Skoog plates in a growth chamber at 22°C under a 16 hr of light and 8 hr of dark cycle. 4-day-old seedlings were dipped in water or S. chinensis extract for 15 hr. Total RNA was isolated and sequenced.

正如典型昆虫虫瘿的观测特征所示，五倍子蚜虫（Schlechtendalia chinensis）的提取物可诱导产生过量或异位的营养组织、维管组织与保护组织。本分析属于基于拟南芥（Arabidopsis）的虫瘿形成检测体系（Ab-GALFA）的组成部分，证实该体系可作为解析虫瘿形成分子机制的模式系统。本实验设置如下培养条件：野生型哥伦比亚株系拟南芥（Col-0）接种于垂直放置的1/2浓度Murashige和Skoog（MS）培养基平板上，在培养箱中于22℃、16小时光照/8小时黑暗的光周期条件下培养。取萌发4天的幼苗，分别浸入清水或五倍子蚜虫提取物中处理15小时。随后提取总RNA并完成测序。