In vitro evidence consistent with an interaction between wild type and a mutant SOD1 protein associated with canine degenerative myelopathy

The data sets are associated with four experimental approaches to investigate a mutant gene that had been cloned into a vector plasmid to generate fluorescent tagged (Cherry or GFP wildtype or mutant SOD1 proteins: a. a collection of fluorescent images of cells to count aggregate positive cells (WT and Mutant (DM) with GFP or Cherry) (JPEGS format) b. A collection of western blots that confirm the expression of the SOD1 fusion protein, the fluorescent protein only and loading control with b-actin. (Tiff format) c. A native gel treated with NBT to display SOD1 activity. Corresponding western blots shown to confirm SOD1 fusion protein expression. (Tiff format) d. Confocal images showing the co-expression of wild type and mutant fusion proteins within cellular aggregates. (Tiff format) e. Western blot of immunoprecipitation (pull down) products supporting the co-precipitation of wildtype and mutant SOD1. (Tiff format)

本数据集配套四种实验方法，用于研究已克隆至载体质粒、以制备荧光标记蛋白（标记物为Cherry或绿色荧光蛋白（Green Fluorescent Protein, GFP），靶蛋白为野生型或突变型超氧化物歧化酶1（Superoxide Dismutase 1, SOD1））的突变基因。
a. 用于计数聚集阳性细胞的细胞荧光图像集，样本包含标记GFP或Cherry的野生型WT与突变型DM组，格式为JPEG；
b. 免疫印迹（Western Blot）数据集，用于验证SOD1融合蛋白、单独荧光蛋白及上样内参β-肌动蛋白的表达情况，格式为Tiff；
c. 经氮蓝四唑（Nitroblue Tetrazolium, NBT）染色以显示SOD1活性的天然凝胶电泳结果，配套免疫印迹用于验证SOD1融合蛋白的表达，格式为Tiff；
d. 共聚焦显微镜图像，展示野生型与突变型融合蛋白在细胞聚集体中的共表达现象，格式为Tiff；
e. 免疫沉淀（Immunoprecipitation, IP，又称下拉实验pull down）产物的免疫印迹结果，证实野生型与突变型SOD1发生共沉淀，格式为Tiff。