Chondroprotective functions of neutrophil-derived extracellular vesicles via promoting the production of secreted frizzled related protein 5 in cartilage

Neutrophil extracellular vesicles (EVs) are known to play a protective role in arthritic joints by suppressing inflammation and cartilage degeneration. In the current study, we investigated the functional characteristics of EVs derived from stimulated neutrophils and their protective effects in cartilage repair in vitro. We first optimized the stimulation conditions of neutrophils that led to production of EVs with chondroprotective function. To understand this protective mechanism, EVs from TGF-β-stimulated neutrophils were added to stimulated chondrocytes with IL-1β, and their responses were examined by bulk-RNA analysis. RNA-seq for human chondrocyte cell line stimulated by IL-1 β and Evs derived from neutrophils

中性粒细胞胞外囊泡（extracellular vesicles, EVs）已知可通过抑制炎症反应与软骨退变，在关节炎关节中发挥保护作用。本研究中，我们探究了活化中性粒细胞来源胞外囊泡的功能特性，及其在体外软骨修复中的保护效应。我们首先优化了可产生具有软骨保护功能胞外囊泡的中性粒细胞活化条件。为阐明该保护机制，我们将经转化生长因子-β（transforming growth factor-β, TGF-β）刺激的中性粒细胞来源胞外囊泡添加至经白细胞介素1β（interleukin-1β, IL-1β）刺激的软骨细胞体系中，通过批量RNA测序分析（bulk-RNA analysis）检测细胞应答情况，分析对象涵盖经IL-1β刺激的人类软骨细胞系及中性粒细胞来源胞外囊泡。