Transcriptional response to TNF-alpha in a panel of 17 human melanoma cell lines

A panel of 17 human melanoma cell lines with known BRAF and NRAS mutation status was stimulated with TNF-alpha for 72 hours. The goal of the study was to correlate the transcriptional response in BRAF versus NRAS mutated melanoma cell lines. Total RNA was obtained from a panel of 17 human melanoma cell lines treated for 72 hours with TNF-alpha or left untreated. Gene expression profiling was done using the Illumina Human HT12 v4 platform.本研究选取17株携带明确BRAF与NRAS突变状态的人黑色素瘤细胞系，以肿瘤坏死因子-α（TNF-alpha）刺激72小时。本研究的核心目标为分析BRAF突变型与NRAS突变型黑色素瘤细胞系的转录应答相关性。总RNA提取自经TNF-alpha刺激72小时或未接受处理的上述17株人黑色素瘤细胞系。研究采用Illumina Human HT12 v4芯片平台完成基因表达谱检测。