An updated proteomic analysis of Drosophila haemolymph after bacterial infection

Using an in-depth Mass Spectrometry-based proteomics approach, we provide a comprehensive characterization of the hemolymphatic proteome of adult flies upon bacterial infection. We detected and quantified changes in abundance of several known immune regulators and effectors, including multiple antimicrobial peptides, peptidoglycan-binding proteins and serine proteases. Comparison to previously published transcriptomic analyses reveals a partial overlap with our dataset, indicating that many proteins released into the haemolymph upon infection may not be regulated at the transcript level. Among them, we identify a set of muscle-derived proteins released into the haemolymph upon infection. Finally, our analysis reveals that infection induces major changes in the abundance of proteins associated with mitochondrial respiration. This study uncovers a large number of previously undescribed proteins potentially involved in the immune response.本研究采用基于质谱（Mass Spectrometry）的深度蛋白质组学方法，对细菌感染后的成年果蝇血淋巴蛋白质组进行了全面表征。我们检测并定量了多种已知免疫调控因子与效应蛋白丰度的变化，包括多种抗菌肽、肽聚糖结合蛋白及丝氨酸蛋白酶。与已发表的转录组学分析结果对比后发现，本数据集与后者存在部分重叠，这表明感染后释放进入血淋巴的诸多蛋白质可能并不受转录水平调控。在此类蛋白中，我们鉴定出了一组感染后释放进入血淋巴的肌肉来源蛋白。最后，本分析揭示感染会显著改变与线粒体呼吸相关的蛋白质丰度。本研究还发现了大量此前未被报道的、可能参与免疫应答的蛋白质。