Transcriptome of lung tissue from C57BL/6 mice with or without neutrophil depletion at ZT04 and ZT16

Our study looks at the dirsruption of lung circadian transcriptome that occurs when neutrophils are depleted (by application of antibodies (anti-Ly6G-1A8) to wildtype C57BL/6 mice, or Diphtheria toxin (DT) to neutrophil-specific DT-susceptible mice (MRP8-Cre;iDTR-flox)). Overall design: Lungs were harvested from neutrophil-depleted (antibody or DT) or non-depleted mice, in normal light-controlled mouse facility (ZT4) or 12h inverted light cabinets (ZT16). Experiments were carried out over 3 batches (July 2017, September 2017, and April 2018), with 3 or 4 mice per group. Antibody-depleted and non-depleted mice were tested for the July 2017 and September 2017 batches, whereas DT-depleted mice were tested only in April 2018.

本研究旨在探究中性粒细胞（neutrophils）缺失时肺昼夜节律转录组（circadian transcriptome）所发生的失调现象。中性粒细胞缺失可通过两种方式实现：一是向野生型C57BL/6小鼠（wildtype C57BL/6 mice）注射抗Ly6G-1A8抗体（anti-Ly6G-1A8），二是向中性粒细胞特异性白喉毒素易感小鼠（neutrophil-specific DT-susceptible mice，即MRP8-Cre;iDTR-flox）注射白喉毒素（Diphtheria toxin, DT）。实验设计如下：在正常光照受控的小鼠饲养设施（光照时点为ZT4）或12小时倒置光照柜（光照时点为ZT16）中，分别采集中性粒细胞缺失组（经抗体或白喉毒素处理）与未缺失对照组小鼠的肺组织。实验共分为3批开展，分别为2017年7月、2017年9月及2018年4月，每组包含3或4只小鼠。其中2017年7月与2017年9月批次仅检测了抗体缺失组与未缺失对照组小鼠，而白喉毒素缺失组小鼠仅在2018年4月批次中完成检测。