Mucin-type O-glycosylation controls pluripotency in mouse embryonic stem cells via Wnt receptor endocytosis. Mucin-type O-glycosylation controls pluripotency in mouse embryonic stem cells via Wnt receptor endocytosis

Mucin-type O-glycosylation regulates mouse embryonic stem cells pluripotency by directly modulating the endocytosis of the Wnt receptor Frizzled-5, finely tuning Wnt signaling outcome. Overall design: RNA-seq to examine galectins and Znrf3/Rnf43 expression in 1 technical and biological replicate of WT R1 mouse ESC cell line

黏蛋白型O-糖基化（Mucin-type O-glycosylation）通过直接调控Wnt受体卷曲蛋白5（Frizzled-5）的内吞作用，精细调控Wnt信号通路的输出效应，进而调控小鼠胚胎干细胞的多能性。
实验设计概述：采用RNA测序（RNA-seq）检测野生型R1小鼠ESC细胞系的1组技术重复与生物学重复样本中半乳糖凝集素（galectins）以及Znrf3/Rnf43的表达水平。