Catalan Surveillance Network of SARS-CoV-2 in Sewage

The Catalan Surveillance Network of SARS-CoV-2 in Sewage started in July 2020 to monitor 56 wastewater treatment plants (WWTPs) all over the country representing the sewage from the 80% of the total population. The sampling plan consist in a weekly collection of 24 hours-composed-flow proportional water sample from the entrance of each WWTPs. The raw sewage samples are send to references laboratories for analysis within 24h after collection. Samples are concentrated, the RNA extracted and finally amplyfied using two target genes (N1/N2 or N1/IP4). Here, we periodically release the dataset of the results obtained for each WWTP analysed with sampling date, gene copies per litre for each viral target, the daily flow from the WWTP, rain events registered and extra comments. Moreover, since November 2021, the network analyses periodically the presence of Variants of Concern (VOCs) in the studied WWTPs. Raw data generated from sequencing is available upon request at [e-mail].加泰罗尼亚新冠病毒（SARS-CoV-2）污水监测网络于2020年7月启动，旨在对全国范围内的56座污水处理厂（wastewater treatment plants，简称WWTPs）开展监测，覆盖全国80%总人口的污水排放范围。该监测网络的采样方案为：每周从每座污水处理厂的进水口采集24小时混合流量比例水样。采集得到的原始污水样本需在24小时内送至指定参考实验室进行分析。样本将先经浓缩处理、提取核糖核酸（RNA），最终以两个靶标基因（N1/N2或N1/IP4）为靶点完成核酸扩增。 本监测网络将定期发布各污水处理厂的检测结果数据集，包含采样日期、每升水样中各病毒靶标的基因拷贝数、污水处理厂的日处理流量、记录的降雨事件以及额外备注信息。 此外，自2021年11月起，该监测网络还定期对所覆盖的污水处理厂中的新冠关切变异株（Variants of Concern，简称VOCs）开展检测。测序产生的原始数据可通过发送邮件至[e-mail]申请获取。