Peripheral blood gene expression as a biomarker for early detection of Alzheimer’s disease. Homo sapiens

To further development of our gene expression profile of Alzheimer's disease, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential biomarker for early disease detection Overall design: Total RNA from peripheral blood cells was extracted, reverse-transcribed and labelled, then analysed for gene expression using GeneSpring GX12 (Agilent Technologies, USA). About 180 samples (AD=90, Non-demented control=90) was used for microarray analysis.

为推进本团队针对阿尔茨海默病（Alzheimer's disease）的基因表达谱研究，本研究采用全基因组微阵列表达谱作为发现平台，筛选具备阿尔茨海默病早期检测潜在生物标志物（biomarker）价值的基因。实验设计：提取外周血细胞总RNA，经反转录、标记后，使用GeneSpring GX12（美国安捷伦科技有限公司，Agilent Technologies）完成基因表达分析。本研究共纳入约180份样本，其中阿尔茨海默病组90份、非痴呆对照组90份，均用于微阵列分析。