Transcriptomic data of CRP(T168P) and AC(S254R) mutant strain of E. coli W

Using a synthetic biosensor to couple production of a specific metabolite with cell growth, we spontaneously evolved cells under the selective condition toward the acquisition of genotypes that optimally reallocated cellular resources. Using 3-hydroxypropionic acid (3-HP) production from glycerol in Escherichia coli as a model system, we determined that spontaneous mutations in the conserved regions of proteins involved in global transcriptional regulation altered the expression of several genes associated with central carbon metabolism. Our study provides a new perspective on adaptive laboratory evolution (ALE) using synthetic biosensors, thereby supporting future efforts in metabolic pathway optimization. Overall design: mRNA profiles of wild-type (WT) and CRP(T168P) or AC(S254R) mutant strain of E. coli W本研究利用合成生物传感器（synthetic biosensor）将特定代谢物的合成与细胞生长进行偶联，在选择性培养条件下自发进化细胞，使其获得最优分配细胞资源的基因型。以大肠杆菌（Escherichia coli）利用甘油生产3-羟基丙酸（3-hydroxypropionic acid，3-HP）作为模型系统，我们发现参与全局转录调控的蛋白质保守区域发生自发突变后，会改变中心碳代谢相关的多个基因的表达水平。本研究为基于合成生物传感器的自适应实验室进化（adaptive laboratory evolution，ALE）提供了全新的研究视角，可为代谢途径优化的后续工作提供理论支撑。整体实验设计：大肠杆菌W株的野生型（wild-type，WT）与CRP(T168P)、AC(S254R)突变菌株的mRNA转录组谱。