human, gastric cancer line, LC-MSMS

We also tested the downstream differentiation of protein expression between BGC823 Ctrl and BGC823 MIR137HG by IP target FUS following LC-MS/MS. And we selected several proteins related to tumor growth and metastasis to analyze further. String data showed that they might have more contact with each other. Western blot data showed that MET, CTNNB1 and RHOC were also co-expressed with the over-expression MIR137HG.

我们还通过免疫沉淀（Immunoprecipitation, IP）靶向融合蛋白FUS，并结合液相色谱-串联质谱（Liquid Chromatography-Tandem Mass Spectrometry, LC-MS/MS），检测了胃癌细胞系BGC823对照组（Ctrl）与过表达MIR137HG的BGC823细胞之间的蛋白质表达下游差异。随后我们筛选了数种与肿瘤生长及转移相关的蛋白质开展进一步分析。STRING互作蛋白数据库（STRING）分析结果显示，这些蛋白质间可能存在更为紧密的相互作用。蛋白质免疫印迹（Western Blot, WB）实验结果表明，MET、CTNNB1以及RHOC均与过表达的MIR137HG存在共表达关系。