Vi impacts on the cytokine profile of innate cells shortly after infection.

Cells were stained with mAb to specific surface markers F4/80+, CD11c+, DX5+/CD3− and Ly6G+ to identify innate populations, permeabilised and stained with anti-MIP-2, TNF-α, IFN-γ, perforin and IL-6. Data represent percent of cytokine positive cells out of total spleen populations ± SEM.*indicates significant values of p**, p***, p†indicates significant values of p††, p†††, p+ are compared to Vi− infected mice.采用针对特异性表面标志物F4/80+、CD11c+、DX5+/CD3−及Ly6G+的单克隆抗体（monoclonal antibody, mAb）对细胞进行染色，以鉴定先天免疫细胞群；随后对细胞进行透化处理，并用抗巨噬细胞炎症蛋白2（macrophage inflammatory protein 2, MIP-2）、肿瘤坏死因子α（Tumor Necrosis Factor α, TNF-α）、干扰素γ（Interferon γ, IFN-γ）、穿孔素及白细胞介素6（Interleukin 6, IL-6）的抗体实施胞内染色。数据以脾脏总细胞群中细胞因子阳性细胞所占百分比±标准误（standard error of the mean, SEM）表示。*代表p**、p***组具有统计学显著性，†代表p††、p†††组具有统计学显著性，所有比较均以Vi⁻感染小鼠为对照。