Whole slide images of pancreatic cross-sections of NOD mice stained with antibodies: CD11c, CD113, DAPI, F4/80, glucagon, insulin and IL-1β.

We provide a dataset of 11 whole-slide images of pancreatic cross-sections of non-obese diabetic (NOD) mice that were stained for CD11c (for myeloid cells), CD3 (for T-cells), DAPI (for nuclei), F4/80 (for macrophages), glucagon (for α-cells), insulin (for β-cells), and IL-1β. We performed 7 color multispectral imaging using the Akoya Biosciences Vectra Polaris instrument under 20X magnification and a pixel size of 0.5 μm/px. We also provide clinical data including the age of surgery, highest blood glucose recording, number of intact islets in the cross section, and the range of insulitis degrees (T-/β-cell count ratios - calculated for each islet).

本数据集包含11幅非肥胖糖尿病（non-obese diabetic, NOD）小鼠胰腺组织切片的全切片图像，上述切片已针对以下标志物完成染色：CD11c（标记髓系细胞）、CD3（标记T细胞）、DAPI（标记细胞核）、F4/80（标记巨噬细胞）、胰高血糖素（标记α细胞）、胰岛素（标记β细胞）以及白细胞介素1β（IL-1β）。本研究采用Akoya Biosciences Vectra Polaris仪器进行7色多光谱成像，成像参数为20倍放大倍率，像素尺寸0.5 μm/px。本数据集同时附带配套临床数据，包括手术时年龄、最高血糖检测值、切片中完整胰岛的数量，以及胰岛炎程度范围（即针对每个胰岛计算得出的T细胞/β细胞计数比值）。