Gene expression profiling of hepatocarcinogenesis initiation in Fischer-344 rats.

Diethylnitrosamine is used as initiator carcinogen in the modiffied resistan model but is not known its effect on gene expression profile on this stage. In other way, CAPE is compound with anticarcinogenic propertie when is administered 12 h before DEN and with the aim to characterize its effect on gene expression profile when is administered alone and in combination with diethylnitrosamine, these analysis were made. Male Fischer-344 rats were we used 4 animal per each treatment and all treatments are compared with not treated rats. Were identified groups of genes related with Carcinogens metabolism, oxidative stress and cell regulation with important differences between treatments.本研究中，改良抗性模型采用二乙基亚硝胺（Diethylnitrosamine，下文简称DEN）作为致癌启动剂，但目前尚不明确其在此模型阶段对基因表达谱的影响。另一方面，咖啡酸苯乙酯（CAPE）是一种具备抗癌特性的化合物，为表征其单独给药及与二乙基亚硝胺联合给药时对基因表达谱的影响，本研究在DEN给药前12小时给予CAPE并开展了相关分析。实验选用雄性Fischer-344大鼠，每组处理设置4只动物，所有处理组均以未处理大鼠作为对照。最终本研究鉴定出多组与致癌物代谢、氧化应激及细胞调控相关的基因，且各处理组间存在显著表达差异。