Recombination and Darwinian selection as drivers of genetic diversity and evolution of sweet potato leaf curl viruses in Tanzania

Using a combination of Next-Generation and Sanger sequencing techniques, we detected sweet potato leaf curl viruses (SPLCVs; Genus Begomovirus) and determined their genetic diversities, recombination, and evolutionary selection pressure imposed on their genes. Eleven complete (2767–2824 nucleotides) and fifty partial (V1 and C1 proteins) sequences of SPLCVs, and one complete genome of sweet potato pakakuyu virus were generated

本研究结合下一代测序技术（Next-Generation sequencing）与桑格测序法（Sanger sequencing），对隶属于菜豆金色花叶病毒属（Begomovirus）的甘薯曲叶病毒（sweet potato leaf curl viruses, SPLCVs）进行了检测，并解析了其遗传多样性、重组特征以及其基因所承受的进化选择压力。本研究共获取11条完整的甘薯曲叶病毒序列（长度为2767~2824个核苷酸）、50条对应V1和C1蛋白的甘薯曲叶病毒部分序列，以及1条甘薯帕卡库病毒（sweet potato pakakuyu virus）的完整基因组。