Additional file 4 of Sampling the fish gill microbiome: a comparison of tissue biopsies and swabs

Additional file 4. UNIFRAC nmMDS. Filtered sequences were aligned using MAFFT [110], and phylogenetic tree built using FASTTREE [111] using default parameters within the QIIME2 pipeline. The resultant UNIFRAC distance matrix was used in generation of non-metric multidimensional scaling analysis as shown here. Results of PERMANOVA using unrestricted permutations for comparison of samples by sample type (swab;biopsy;environmental) shown in table indicate significant variation by sample type.附加文件4：基于UniFrac的非度量多维标度分析结果。经过滤的序列通过MAFFT [110]完成比对，并在QIIME2分析流程中采用默认参数，借助FASTTREE [111]构建系统发育树。所得UniFrac距离矩阵被用于生成非度量多维标度分析，结果如本文件所示。采用无限制置换的置换多元方差分析（PERMANOVA）按样本类型（拭子、活检样本、环境样本）对样本进行组间比较，如表中结果所示，不同样本类型间存在显著差异。