Escherichia coli strain:CS04 Genome sequencing and assembly. Escherichia coli strain:CS04

Whole genome sequencing of multi-drug resistant isolates of commensal Escherichia coli was used to gain insight into the pool of resistance genes in this component of the human gut microbiome, and potential mechanisms by which these genes are spread. Strains of E. coli were isolated from rectal swabs of healthy college students, and tested for antibiotic resistance using disk diffusion assays. The six isolates sequenced were independently isolated, and exhibited high-level resistance to at least three major classes of antibiotics that are ordinarily effective against Gram-negative species.

本研究通过对共生多重耐药大肠埃希菌（Escherichia coli）分离株开展全基因组测序，旨在深入解析该人类肠道微生物组组分所携带的耐药基因库，并阐明此类耐药基因的潜在传播机制。研究人员从健康大学生的直肠拭子中分离得到大肠埃希菌菌株，并采用纸片扩散法药敏试验检测其抗生素耐药性。本次测序的6株分离株均为独立分离所得，且对至少三类通常对革兰氏阴性菌具有抗菌活性的抗生素表现出高水平耐药性。