Microbiome_9

Introduction: Cockroaches (Phylum Arthropoda; Order Blattodea) are resilient insects and capable of surviving in extreme environments and conditions. Host-microbe interactions play a key role in host physiology and may be important in host adaptation to environmental changes. This study aimed to assess the microbial diversity and composition of cockroaches sampled from a very cold environment (kitchen freezer) and typical habitats (sewer and laboratory).Methodology: Cockroach samples were collected from three habitats (a kitchen freezer, a building sewer, and a laboratory). Sequencing of the PCR-amplified 16S V3-V4 hypervariable rRNA region was performed on an Illumina MiSeq platform. The microbial abundance and diversity were assessed.Results: We observed a significant decrease in microbial diversity and taxa species associated with cold temperatures among freezer cockroaches. Freezer cockroaches had unique microbial communities, different from the sewer and laboratory cockroach populations but shared a few taxa with the sewer cockroaches.Conclusions: Our results suggest that the environment may shape the microbial composition of organisms. Further, our study demonstrated the association of cold-resistant microbes with cockroaches collected from a kitchen freezer.引言：蜚蠊（节肢动物门(Arthropoda)；蜚蠊目(Blattodea)）是一类抗逆性极强的昆虫，能够在极端环境与条件下存活。宿主-微生物互作在宿主生理学过程中发挥关键作用，且可能对宿主适应环境变化具有重要意义。本研究旨在评估分别从极寒环境（厨房冰柜）与典型生境（下水道与实验室）采集的蜚蠊的微生物多样性与群落组成。 研究方法：本研究从三类生境（厨房冰柜、建筑下水道及实验室）采集蜚蠊样本。采用Illumina MiSeq测序平台对经PCR扩增的16S rRNA基因V3-V4高变区（16S rRNA V3-V4 hypervariable region）进行测序，并对微生物的丰度与多样性进行分析。 结果：研究发现，冰柜内蜚蠊的微生物多样性与相关类群的丰度随低温环境呈现显著下降趋势。冰柜蜚蠊拥有独特的微生物群落结构，与下水道及实验室蜚蠊种群存在显著差异，但与下水道蜚蠊共享少量分类类群。 结论：本研究结果表明，环境可塑造生物体的微生物群落组成。此外，本研究证实了从厨房冰柜采集的蜚蠊体内存在与耐寒性相关的微生物类群。