Effects of peptidomimetic PD00 on OVA-induced immune cells and their metabolic pathways in mouse lung tissue by single-cell transcriptome sequencing. Effects of peptidomimetic PD00 on OVA-induced immune cells and their metabolic pathways in mouse lung tissue by single-cell transcriptome sequencing

OVA-induced allergic asthma in mice shares many similar features with human asthma. The peptidomimetic PD00 can significantly improve OVA-induced asthma symptoms in mice. However, the molecular mechanism by which it works is still unclear. The metabolic status of immune cells affects their functional status, so single-cell transcriptome sequencing was used to explore the effect of PD00 on immune cell subsets and their metabolic pathways in OVA-treated mouse lung tissue. Overall design: Three samples from mouse lung tissue

卵清蛋白（OVA）诱导的小鼠过敏性哮喘与人类哮喘存在诸多相似特征。肽模拟物（peptidomimetic）PD00可显著改善OVA诱导的小鼠哮喘症状。然而其发挥作用的分子机制仍未明确。免疫细胞的代谢状态会影响其功能状态，因此本研究采用单细胞转录组测序（single-cell transcriptome sequencing）技术，探究PD00对经OVA处理的小鼠肺组织中免疫细胞亚群及其代谢通路的影响。实验整体设计：小鼠肺组织来源的3份样本。