Exon Array expression data from 13 areas of the late second trimester human brain. Homo sapiens

Tissue was microdissected from 13 regions, including 9 distinct neocortical areas, from both left and right sides of four late second trimester human brain specimens. Gene- and exon-level differential expression analyses were performed by mixed model, nested analysis of variance using the XRAY software from Biotique Systems. Further details available in Johnson, Kawasawa, et al., "Functional and Evolutionary Insights into Human Brain Development through Global Transcriptome Analysis" Neuron, Volume 62, Issue 4, 2009 Keywords: gene- and exon-level analyses Overall design: For initial analysis, all neocortex samples were grouped together. In subsequent analyses, neocortex areas were compared with each other. In most analyses reported, left and right side samples were treated as additional biological replicates.本研究从4例妊娠中期晚期的人类大脑标本的左右两侧，通过显微切割获取了13个区域的组织，其中包含9个独立的新皮质（neocortex）区域。 采用Biotique Systems公司的XRAY软件，通过混合模型与嵌套方差分析，完成了基因水平及外显子（exon）水平的差异表达分析。 更多详细信息可参阅Johnson、Kawasawa等人发表于《神经元》（Neuron）2009年第62卷第4期的论文《通过全局转录组分析解析人类大脑发育的功能与进化特征》。 关键词：基因与外显子水平分析 实验整体设计：初始分析阶段，将所有新皮质样本合并为一组；后续分析阶段，则对各新皮质区域开展两两比较。在已报道的多数分析中，左右两侧样本被视为额外的生物学重复。