Oxidative stress-mediated alterations of the microRNA expression profile in porcine granulosa cells using RNA-Seq technique

To investigate the effects of oxidative stress induced by hydrogen peroxide(H2O2) on miRNA expression in porcine granulosa cells,we examined the impact of H2O2-mediated oxidative stress in procine GC through miRNA-Seq. We identified 33 (19 upregulated, 14downregulated) and 22 (14 upregulated, 8 downregulated miRNAs) differentially expressed miRNAs (DEmiRNAs) at 300µM and 100µM H2O2, respectively, compared with the control group. Overall design: miRNA profiles of control and H2O2 treatment groups (100 and 300µM) were generated by deep sequencing using Illumina HiSeqTM 2500