Oxidative stress-mediated alterations of the microRNA expression profile in porcine granulosa cells using RNA-Seq technique

To investigate the effects of oxidative stress induced by hydrogen peroxide(H2O2) on miRNA expression in porcine granulosa cells,we examined the impact of H2O2-mediated oxidative stress in procine GC through miRNA-Seq. We identified 33 (19 upregulated, 14downregulated) and 22 (14 upregulated, 8 downregulated miRNAs) differentially expressed miRNAs (DEmiRNAs) at 300µM and 100µM H2O2, respectively, compared with the control group. Overall design: miRNA profiles of control and H2O2 treatment groups (100 and 300µM) were generated by deep sequencing using Illumina HiSeqTM 2500为探究过氧化氢（hydrogen peroxide, H₂O₂）诱导的氧化应激对猪颗粒细胞（porcine granulosa cells）中微小RNA（microRNA, miRNA）的表达影响，本研究通过微小RNA测序（miRNA-Seq）分析了过氧化氢介导的氧化应激对猪颗粒细胞的作用效果。与对照组相比，分别在300μM和100μM过氧化氢处理组中鉴定出33个（19个上调、14个下调）和22个（14个上调、8个下调）差异表达微小RNA（differentially expressed miRNAs, DEmiRNAs）。研究整体设计：采用Illumina HiSeq™ 2500平台进行深度测序，获取对照组与过氧化氢处理组（100μM和300μM）的微小RNA表达谱。