Transcriptome profiling of HepG2 cells in Cdc20 overexpressed and downregulated condition

Cdc20 acts as a regulatory protein interacting with many other proteins at multiple points in the cell cycle. Here we tried to explore the novel transcription regulatory function of Cdc20 by means of altering Cdc20 level in HepG2 cells. We transiently over expressed Flag tagged Cdc20 (F) and siRNA mediated knocked down Cdc20(S) in HepG2 cell line and performed whole genome microarray analysis using illumina BeadChip Array. Significantly altered genes were subjected to the analysed by Ingenuity pathway analysis software (Build version: 302937) and crucial cellcycle and apoptotic pathways were found to be altered. These pathways could further be explored as anticancer therapy in liver carcinoma. Identification of potential signature genes in Cdc20 altered condition in hepatocellular carcinoma cells细胞分裂周期蛋白20（Cdc20）是一类调控蛋白，可在细胞周期的多个节点与多种其他蛋白发生相互作用。本研究通过改变HepG2细胞中Cdc20的表达水平，旨在探究其全新的转录调控功能。我们在HepG2细胞系中分别实施两组处理：瞬时过表达Flag标签融合的Cdc20（F组），以及通过小干扰RNA（siRNA）介导敲低Cdc20（S组），随后采用Illumina BeadChip芯片阵列开展全基因组微阵列分析。对显著差异表达的基因，我们使用Ingenuity通路分析软件（构建版本：302937）进行解析，发现关键的细胞周期与细胞凋亡通路发生了显著改变。上述通路可进一步作为肝细胞癌抗癌治疗的潜在靶点开展深入研究。本研究还鉴定了肝细胞癌细胞中Cdc20表达改变条件下的潜在特征基因。