Whole Blood RNA Expression in Pink1-/- and Wildtype Rats. Whole Blood RNA Expression in Pink1-/- and Wildtype Rats

Early-onset pathology and reliable disease biomarkers for diagnostics are poorly understood for progressive degenerative disorders such as Parkinson disease (PD). PD is the second most common age-related degenerative disorder. The Pink1-/- rat is a mitochondrial dysfunction model of prodromal PD. In this study, we used RNA sequencing to examine gene expression within whole blood samples of young and old Pink1-/- rats as compared to age-matched wildtype controls. Overall design: Comparative gene expression profiling analysis using data obtained from the RNA-sequencing between the two genotypes (Pink1-/- and WT) in both young and old rats.

对于帕金森病（Parkinson disease, PD）这类进行性退行性疾病，其早期发病病理与可用于临床诊断的可靠疾病生物标志物，目前尚未得到充分阐明。帕金森病是第二大常见的年龄相关性退行性疾病。Pink1基因敲除大鼠（Pink1-/- rat）是前驱期帕金森病的线粒体功能障碍模型。本研究采用RNA测序（RNA sequencing）技术，对年轻与老年Pink1基因敲除大鼠及其同年龄匹配的野生型对照的全血样本基因表达水平进行检测分析。实验整体设计：以年轻及老年大鼠的两种基因型（Pink1-/-与野生型（Wildtype，缩写WT））为研究对象，利用RNA测序所得数据开展对比基因表达谱分析。