Next Generation Sequencing Facilitates Quantitative Analysis of Honey Bee Brain Transcriptomes Treated by Dinotefuran

Purpose：The goals of this study are to obtain NGS-derived honey bee brain transcriptome profiling (RNA-seq) after dinotefuran treatment in honey bee Methods：Through deep sequencing in triplicate, using Illumina Hiseq2500,a comprehensive analysis of lncRNAs and mRNAs was performed following persistent exposure to 0.01 mg/L dinotefuran for 1, 5, and 10 d. Results： In total, 312 lncRNAs and 1341 mRNAs, 347 lncRNAs and 1458 mRNAs, 345 lncRNAs and 1155 mRNAs were found to be differentially expressed (DE) on days 1, 5 and 10 respectively. Conlusions：This study characterized the expression profile of lncRNAs upon exposure to neonicotinoid insecticides in young adult honey bees. Honey bee brain lncRNA profiles of 1-day old, 5-day-old and 10-day-old dinotefuran treated groups and control groups研究目的：本研究旨在获取蜜蜂经呋虫胺（dinotefuran）处理后，基于下一代测序（NGS，Next-Generation Sequencing）得到的大脑转录组谱（RNA测序，RNA-seq）数据。 研究方法：本研究采用伊卢米纳HiSeq2500（Illumina Hiseq2500）测序平台开展三次生物学重复深度测序，对持续暴露于0.01 mg/L呋虫胺1天、5天及10天的蜜蜂样本中的长链非编码RNA（lncRNAs）与信使RNA（mRNAs）进行全面分析。 研究结果：总计在处理第1天、第5天和第10天，分别检测到312个长链非编码RNA与1341个信使RNA、347个长链非编码RNA与1458个信使RNA、345个长链非编码RNA与1155个信使RNA呈现差异表达（DE）。 研究结论：本研究阐明了年轻成年蜜蜂暴露于新烟碱类杀虫剂（neonicotinoid insecticides）后长链非编码RNA的表达谱特征，涵盖了1日龄、5日龄及10日龄呋虫胺处理组与对照组的蜜蜂大脑长链非编码RNA表达谱数据。