ERK1/2-RSK2 signaling is a developmental switch required for estrogen homeostasis

Transcriptomic analysis of the mature ER+ population in the mammary gland revealed enrichment for estrogen-responsive genes in the RSK2-KO compared to the wild type. Anlaysis of gene expresison from FACS sorted EpCam-hi_CD49f+lowSca+CD49b- cells from the mammary glands of 12wk RSK2(+/+) or RSK2(-/-) mice at estrous and diestrus stages.

对乳腺中成熟雌激素受体阳性（ER+）细胞群开展的转录组学分析（Transcriptomic analysis）显示，相较于野生型（wild type），RSK2敲除（RSK2-KO）样本中雌激素应答基因（estrogen-responsive genes）的富集水平显著更高。本研究针对动情期（estrous）与动情间期（diestrus）的12周龄RSK2野生型纯合子（RSK2(+/+)）及敲除纯合子（RSK2(-/-)）小鼠的乳腺组织，对经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）分离得到的EpCam-hi_CD49f+lowSca+CD49b-细胞的基因表达情况展开了分析。