遇见数据集

Summary statistics for the assembly and annotation of the Gryllodes sigillatus male accessory gland transcriptome.

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Figshare2015-12-03 更新2026-04-29 收录
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aQC stands for quality control. This step primarily removes reads of poor sequencing quality after base calling. It also removes from reads those sequences that correspond to adapters and are used to construct the normalized cDNA library as well as those added for the sequencing itself.bBLASTx was used to annotate the transcriptome against the non-redundant protein database at NCBI, with an expected value cutoff of 10−6.Summary statistics for the assembly and annotation of the Gryllodes sigillatus male accessory gland transcriptome.

aQC即质量控制(quality control)。该步骤主要在碱基识别(base calling)流程完成后,移除低测序质量的读段(reads);同时从读段中剔除两类序列:一类是用于构建标准化cDNA文库的接头(adapter)序列,另一类是为测序流程本身添加的序列。 本研究使用bBLASTx工具,以10⁻⁶作为期望值(E-value)阈值,将转录组与美国国家生物技术信息中心(National Center for Biotechnology Information,NCBI)的非冗余蛋白数据库进行比对注释。 本数据集包含Gryllodes sigillatus雄性附腺转录组的组装与注释统计汇总结果。

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2015-12-03
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