High-throughput sequencing and analysis of gene modification regulated by epigenetics modification in adult and old intracerebral hemorrhage rats. [ChIP-Seq]. High-throughput sequencing and analysis of gene modification regulated by epigenetics modification in adult and old intracerebral hemorrhage rats. [ChIP-Seq]

Here we performed in depth epigenome sequencing and analysis to study the mechanism of high mortality rate of ICH in rat brain. It reveals a systematical transcription activation of mRNA genes enriched in inflammation and immune responses. These genes are clustered to specific genomic loci and regulated by repressive and activation chromatin markers. We show that epigenetic reprogramming responding to ICH in old rat brains differs greatly from that in the adult. Overall design: Examination of 3 different histone modifications in adult (13 mon old) and old (22 mon old) ipsilateral and contralateral brain tissues，2 different histone modifications in old ipsilateral brain tissues with young plasma and old plasma treatment

本研究开展深度表观基因组测序（epigenome sequencing）与分析，旨在探究大鼠脑内脑出血（Intracerebral Hemorrhage, ICH）高死亡率的作用机制。本研究发现，大量富集于炎症与免疫应答过程的mRNA基因发生系统性转录激活。这些基因聚集于特定基因组位点，并受抑制性与激活性染色质标记（chromatin markers）调控。我们发现，老年大鼠脑内响应ICH的表观遗传重编程（epigenetic reprogramming）过程与成年大鼠存在显著差异。总体实验设计：对成年（13月龄）与老年（22月龄）大鼠的同侧（ipsilateral）及对侧（contralateral）脑组织开展3种不同组蛋白修饰（histone modifications）的检测；对经年轻血浆与老年血浆处理的老年同侧脑组织开展2种不同组蛋白修饰的检测。