Expression profiles of circular RNA in A549 cells infected by Influenza virus

Circular RNAs (circRNAs) participate in regulating many biological processes. However, their roles in influenza A virus (IAV) pathogenicity are largely unknown. Here, we analyzed the expression profile of circRNAs in the H1N1-infected cells by high-throughput sequencing Overall design: Expression profiles of circRNAs in A549 cells infected by A/Puerto Rico/8/34 (H1N1) were generated by deep sequencing,in triplicate, using Illumina Hiseq 3000.环状RNA（Circular RNAs, circRNAs）可参与调控多种生物学进程，但其在甲型流感病毒（Influenza A virus, IAV）致病性中的作用目前仍未明确。本研究通过高通量测序技术，分析了H1N1病毒感染细胞内的环状RNA表达谱。实验设计：以感染A/波多黎各/8/34（H1N1）毒株的A549细胞为材料，采用Illumina Hiseq 3000平台开展深度测序，设置三次生物学重复以获取环状RNA表达谱数据。