Transcriptomic analysis of human breast and prostate cancer cell lines on lysophosphatidic acid (LPA) stimulation

LPA is a natural bioactive lipid with growth factor-like functions due to activation of series of six G protein-coupled receptors (LPA1-6). In this study we determine the LPA induced early-gene expression profile in three unrelated human cancer cell lines (MDA-MB-231, MCF7, PC3) with an objective to identify potential biomarker/s specifically upregulated through the activation of LPA receptor type 1 (LPA1) MDA-MB-231, MCF7, PC3 cells were serum starved for 24h and then stimulated with LPA (1µM) for 45min. The controls were the serum starved unstimulated cells. Two replicates for each sample were included in this study. Microarray analysis was performed using a high-density oligonucleotide array (GeneChip Human Genome U133 plus 2.0 array, Affymetrix)溶血磷脂酸（LPA）是一种天然生物活性脂质，因其可激活六种G蛋白偶联受体（G protein-coupled receptor, GPCR）亚型LPA1至LPA6，故而具备类生长因子功能。本研究以三株无关人癌细胞系（MDA-MB-231、MCF7、PC3）为研究对象，分析LPA诱导的早期基因表达谱，旨在鉴定经LPA1型受体（LPA1）激活后特异性上调的潜在生物标志物。本研究中，将MDA-MB-231、MCF7、PC3细胞进行24小时血清饥饿培养，随后以1μM浓度的LPA刺激45分钟；对照组为仅经24小时血清饥饿但未接受LPA刺激的细胞。每个样本均设置两份重复样本。本研究采用高密度寡核苷酸芯片（GeneChip Human Genome U133 plus 2.0 array，Affymetrix）开展微阵列分析（microarray analysis）。