Supplemental figure3: REP, CTL:AIM-V, ICM, TAC

Investigate the influence of activation versus media on function (TNFa) and mitochondrial mass (mitotracker green) on day 14 of expansion. REP was compared to CA5(CTL:AIM-V) icm stim and CA5 tac stim for the comparison of different activation methods within the same media. CA5, ICM and TAC media all stimulated with icm was a comparison of the influence of media. This was repeated (CA5, ICM and TAC media) but with tac stimulation. icm- Stemcell technologies CD3/CD28 activators tac - Miltenyi TransACT CD3/CD28 activators
Notes:
The mitochondrial mass and function(TNFa) of the T cell product was influenced by the media.