Supplemental figure3: REP, CTL:AIM-V, ICM, TAC

Investigate the influence of activation versus media on function (TNFa) and mitochondrial mass (mitotracker green) on day 14 of expansion. REP was compared to CA5(CTL:AIM-V) icm stim and CA5 tac stim for the comparison of different activation methods within the same media. CA5, ICM and TAC media all stimulated with icm was a comparison of the influence of media. This was repeated (CA5, ICM and TAC media) but with tac stimulation. icm- Stemcell technologies CD3/CD28 activators tac - Miltenyi TransACT CD3/CD28 activators Notes: The mitochondrial mass and function(TNFa) of the T cell product was influenced by the media.本研究旨在探究细胞扩增第14天时，激活方式与培养基对T细胞功能（肿瘤坏死因子α，TNFα）及线粒体质量（Mitotracker Green）的影响。为比较同一培养基下不同激活方式的效果，本研究将REP组、CA5(CTL:AIM-V)培养基的ICM刺激组及CA5培养基的TAC刺激组进行了对比。以ICM刺激CA5、ICM及TAC三种不同培养基的实验组，用于评估培养基类型对实验结果的影响。本研究针对CA5、ICM及TAC三种培养基，采用TAC刺激方式重复了上述培养基影响的对比实验。试剂说明：ICM激活试剂为Stemcell Technologies公司的CD3/CD28激活剂；TAC激活试剂为Miltenyi公司的TransACT CD3/CD28激活剂。备注：T细胞产物的线粒体质量与功能（TNFα）受培养基类型的影响。