Supplementary information files for Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe

Supplementary files for article Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe. Peroxynitrite (ONOO−) is a powerful and short-lived oxidant formed in vivo, which can react with most biomolecules directly. To fully understand the roles of ONOO− in cell biology, improved methods for the selective detection and real-time analysis of ONOO− are needed. We present a water-soluble, luminescent europium(III) probe for the rapid and sensitive detection of peroxynitrite in human serum, living cells and biological matrices. We have utilised the long luminescence lifetime of the probe to measure ONOO− in a time-resolved manner, effectively avoiding the influence of autofluorescence in biological samples. To demonstrate the utility of the Eu(III) probe, we monitored the production of ONOO− in different cell lines, following treatment with a cold atmospheric plasma device commonly used in the clinic for skin wound treatment.本数据集为论文《基于反应型镧系探针的过氧亚硝基阴离子时间分辨发光检测》的补充文件。过氧亚硝基阴离子（Peroxynitrite，ONOO−）是一种在生物体内生成的强氧化性且寿命极短的活性氧化剂，可直接与绝大多数生物分子发生反应。为全面阐明过氧亚硝基阴离子在细胞生物学中的功能角色，亟需开发具备高选择性检测与实时分析能力的新型分析方法。本研究报道了一种水溶性发光型铕(III)探针（europium(III) probe），可在人血清、活细胞及生物基质（biological matrices）中实现过氧亚硝基阴离子的快速灵敏检测。我们利用该探针较长的发光寿命，以时间分辨模式实现过氧亚硝基阴离子的定量检测，有效规避了生物样本中自发荧光（autofluorescence）的背景干扰。为验证该铕(III)探针的实用价值，我们借助临床中常用于皮肤创面治疗的冷大气等离子体装置（cold atmospheric plasma device）处理不同细胞系，并实时监测了其中过氧亚硝基阴离子的生成过程。