Transcriptomic changes in response to different carbon sources in Neurospora crassa

Purpose: We explore gene expression changes when Neurospora crassa wild type responds to different carbon sources in Vogel's medium. Method: We obtained mRNA samples of Neurospora crassa WT in Vogel's minimal medium (VMM) with different carbon source and used RNA-seq technique to measure the trancriptome changes. Results: We identified many genes of transcription factors and enzymes that were up regulated or down regulated in response to the different carbon stimulation. Conclusion: Our data represents a systematic transcriptome profiling of filamentous fungi on different carbon source and identify COL-26 as a critical regulator in degradation of starch components. mRNA profiles of cultures of WT after 4 hours of exposure to media of interest were generated by deep sequencing, in triplicate, using Illumina HiSeq2000. Soluble carbon sources are 2 mM and insoluble carbon source are 2% (w/v).研究目的：本研究旨在探究粗糙脉孢菌（Neurospora crassa）野生型在Vogel培养基中响应不同碳源时的基因表达变化。实验方法：我们从添加不同碳源的Vogel基本培养基（Vogel's minimal medium, VMM）中获取粗糙脉孢菌野生型的mRNA样本，并采用RNA测序（RNA-seq）技术检测转录组表达变化。实验结果：我们鉴定出大量转录因子及酶类编码基因，这些基因在响应不同碳源刺激时呈现上调或下调表达。研究结论：本数据集完成了丝状真菌在不同碳源条件下的系统性转录组谱分析，并鉴定出COL-26作为淀粉组分降解过程中的关键调控因子。本研究通过Illumina HiSeq2000平台开展深度测序，设置三次生物学重复，获取了经目标培养基处理4小时后的野生型菌株培养物的mRNA表达谱。可溶性碳源浓度为2 mM，不溶性碳源浓度为2%（质量体积比，w/v）。