Hierarchical Multi-Omics Remodeling During the Development of Bemisia tabaci

This project investigates the molecular regulation of developmental transitions in the whitefly Bemisia tabaci using an integrative multi omics approach. We generated small RNA sequencing (miRNA), mRNA sequencing (transcriptome), and proteomics datasets across seven key life stages: egg, instar I, instar II, instar III, instar IV, pupa, and adult. Differential expression analyses revealed stage specific regulation involving conserved and novel miRNAs, validated mRNA targets, and corresponding protein changes. Functional annotation (GO and KEGG) demonstrated enrichment in metabolic activation, cuticle remodeling, autophagy, and adult ecological specialization. Stem loop qPCR confirmed miRNA trajectories, and FISH validated miRNA mRNA colocalization. Together, this dataset provides a comprehensive resource for understanding the regulatory architecture of insect development.本研究采用整合多组学（multi omics）策略，探究烟粉虱（Bemisia tabaci）发育转变的分子调控机制。我们针对卵、一龄若虫、二龄若虫、三龄若虫、四龄若虫、蛹及成虫这七个关键生命阶段，构建了小RNA测序（small RNA sequencing，miRNA）、mRNA测序（转录组，transcriptome）以及蛋白质组学（proteomics）数据集。差异表达分析揭示了阶段特异性调控机制，涉及保守型及新型微小RNA（miRNA）、已验证的mRNA靶标以及相应的蛋白质表达变化。基因本体论（Gene Ontology，GO）与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）功能注释结果显示，相关通路显著富集于代谢激活、表皮重塑、自噬及成虫生态特化过程。茎环定量聚合酶链反应（stem loop qPCR）验证了miRNA的表达动态轨迹，荧光原位杂交（FISH）则证实了miRNA与mRNA的共定位现象。综上，本数据集为解析昆虫发育的调控网络架构提供了全面的研究资源。