BACH1 alters the expression of EMT-related genes to promote metastasis in pancreatic cancer [ChIP-Seq]

BTB and CNC homology 1 (BACH1) has been implicated in RAS-driven tumor formation. We focused on the role of BACH1 in Pancreatic ductal adenocarcinoma (PDAC), more than 90% of which have KRAS mutation. BACH1 directly or indirectly repressed the expression of genes for epithelial cell adhesion in AsPC-1 cells and SW1990. Knockdown and overexpression of BACH1 in PDAC cell lines indicated that BACH1 promoted cell migration and invasion in part by reducing E-cadherin expression. Overall design: Search of BACH1 target genes in pancreatic cancer cells

BTB和CNC同源蛋白1（BTB and CNC homology 1, BACH1）已被证实与RAS驱动的肿瘤发生密切相关。本研究聚焦于BACH1在胰腺导管腺癌（Pancreatic ductal adenocarcinoma, PDAC）中的作用——该类肿瘤中超过90%存在KRAS突变。研究人员在AsPC-1与SW1990细胞系中发现，BACH1可直接或间接抑制上皮细胞黏附相关基因的表达。通过在胰腺导管腺癌细胞系中对BACH1进行基因敲低与过表达实验，结果显示BACH1可通过降低E-钙粘蛋白（E-cadherin）的表达，部分促进细胞迁移与侵袭能力。实验整体设计：筛选胰腺癌细胞内的BACH1靶基因。