Proteomic analysis of osteocyte line (MLO-Y4) with knockout of cystic fibrosis transmembrane conductance regulator (CFTR) or chloride deprivation treatment

Mass spectrometry–based proteomic analysis of an osteocyte line, MLO-Y4, was conducted to profile global protein expression changes under two experimental settings, 1) CRISPR-Cas9-based knockout of CFTR versus wild-type; 2) Cl- deprivation versus normal extracellular medium.

本研究针对骨细胞系MLO-Y4开展基于质谱（Mass spectrometry）的蛋白质组学分析，以解析两种实验条件下的全局蛋白质表达变化：1）基于CRISPR-Cas9的CFTR敲除组与野生型对照组；2）氯离子剥夺组与正常细胞外培养液组。