Microbial biopsy data

The data is obtained by flow cytometry after DAPI and Syto9-PI staining for biopsy project. The biopsy technique uncovered significant heterogeneity among the cells within bacterial colonies, identifying differences in cell cycle stages, the proportion of living and dead cells, and the abundance of spore types. Cells from different biopsy sites displayed distinct physiological states, revealing that colony structure is far more complex than previously understood. The technique's precision depends on the biopsy tool size, dye equilibration, and cell handling, underscoring the importance of method calibration. The biopsy method, combined with cytometric fingerprinting, provided insights within only 15 to 45 minutes and is universally applicable.本数据集源自活检项目，经4',6-二脒基-2-苯基吲哚（DAPI）与Syto9-PI染色后，通过流式细胞术（flow cytometry）获取所得。 该活检技术可揭示细菌菌落内细胞间的显著异质性，能够区分细胞周期阶段、活死细胞比例以及孢子类型的丰度差异。 取自不同活检位点的细胞呈现出截然不同的生理状态，这表明菌落结构远比此前学界认知的更为复杂。 该技术的精准度取决于活检工具尺寸、染料平衡条件与细胞处理流程，凸显了方法校准的重要性。 此活检方法与流式细胞指纹图谱（cytometric fingerprinting）相结合后，仅需15至45分钟即可获取分析结果，且具备普适性。