18SrRNA gene diversity at St.S1. seawater metagenome

Seawater was collected from surface to abyssopelagic zone throughout a year at St. S1 (30N, 145E) in 2010-2011. Samples for DNA analysis from each depth were sequentially size fractionated through a 3 um pholycabonate filters (Whatman, Maidstone, UK) followed by filtration onto a 0.2 um Millipore Sterivex filter unit (EMD Milipore, Dermstadt, Germany). The filter samples were frozen immediately and stored at -80 ?C until onshore analysis. Total DNA was extracted and partial 18S rRNA gene of eukaryotes was amplified and sequenced using Illumina Miseq.2010—2011年，研究人员于S1站位（30°N，145°E）开展为期一年的海水样品采集，覆盖从表层至深渊带的全水层。各深度层的DNA分析样品先经3 μm聚碳酸酯滤膜（Whatman，英国梅德斯通）进行逐级粒径分级过滤，随后再通过0.2 μm密理博（Millipore）Sterivex过滤单元（EMD Millipore，德国达姆施塔特）完成过滤。过滤得到的滤膜样品即刻冷冻，保存于-80℃环境直至送至陆地实验室开展分析。实验人员提取样品总DNA，采用Illumina MiSeq测序平台对真核生物的18S rRNA基因片段进行扩增与测序。