Human serum/plasma miScript miRNA PCR array: profiling circulating microRNAs in pregnancies

To identify differentially expressed miRNAs, serum miRNAs were profiled using the Human serum/plasma miScript miRNA PCR array (n=4 per group). The expression of miR-19b-3p was 6-fold (p=0.033) lower in women with GDM compared to controls, miR-20a-5p was 4.5-fold (p=0.047) lower in women with T1DM compared to controls and the expression of miR-29a-3p was 1.8-fold (p=0.002) higher in women with T2DM compared to controls. Differential expression of additional nine miRNAs, although not statistically significant (miR-155-5p, miR-222-3p, miR-9-5p, miR-124-3p, miR-126-3p, miR-210-3p, miR-30d-5p, miR-27a-3p and miR-128-3p) was demonstrated. These differentially expressed miRNAs will be measured in serum and urine samples using SYBR green individual primer assays为鉴定差异表达的微小RNA（miRNAs），本研究采用人血清/血浆miScript miRNA聚合酶链式反应阵列对血清miRNAs进行表达谱分析（每组样本量为4）。与对照组相比，妊娠糖尿病（Gestational Diabetes Mellitus, GDM）女性血清中miR-19b-3p的表达水平下调6倍（p=0.033）；1型糖尿病（Type 1 Diabetes Mellitus, T1DM）女性血清中miR-20a-5p的表达水平下调4.5倍（p=0.047）；2型糖尿病（Type 2 Diabetes Mellitus, T2DM）女性血清中miR-29a-3p的表达水平上调1.8倍（p=0.002）。另有9种miRNAs（miR-155-5p、miR-222-3p、miR-9-5p、miR-124-3p、miR-126-3p、miR-210-3p、miR-30d-5p、miR-27a-3p及miR-128-3p）虽未达到统计学显著性差异，但其表达差异亦得到验证。后续将采用SYBR Green个体引物分析法，对血清及尿液样本中的上述差异表达miRNAs进行检测。