Evaluation of Statistical Methods for Normalization and Differential Expression in mRNA-Seq Experiments. Homo sapiens

A study to evaluate the effect of flowcell and library preparation on the results of transcriptome sequencing using the Illumina Genome Analyzer. The study has two components. (1) Two different samples were spread over 2 different flowcells. We obtained data with and without using the phiX control lane. (2) One sample was prepared in four different library preparations that were spread over 2 different flowcells.本研究旨在评估使用Illumina基因组分析仪（Illumina Genome Analyzer）进行转录组测序（transcriptome sequencing）时，流动槽（flowcell）与文库制备（library preparation）对测序结果的影响。本研究包含两个实验部分：(1) 将两份不同样本分配至两个不同的流动槽中，获取了使用与未使用phiX对照泳道（phiX control lane）的测序数据；(2) 对同一份样本开展四种不同的文库制备操作，随后将制备得到的文库分配至两个不同的流动槽中。