Legionella pneumophila cells after sub-lethal heat shock. Legionella pneumophila cells after sub-lethal heat shock

Legionella pneumophila Philadelphia-1 strain was grown to stationary phase in AYE broth and starved in freshwater for 2 hours and RNA was harvested with or without sublethal heat shock via immersion in a 55 degree C hot water bath for 5 minutes Overall design: Legionella pneumophila Philadelphia-1 strain with or without sublethal heat shock

嗜肺军团菌（Legionella pneumophila）费城-1株（Philadelphia-1 strain）先在AYE肉汤中培养至稳定期，随后于淡水环境中饥饿处理2小时；随后将菌体分别浸入55℃热水浴5分钟以施加亚致死热激，或不施加该处理，随后收集对应的RNA样本。实验设计概述：包含施加或未施加亚致死热激的嗜肺军团菌费城-1菌株样本。