Additional file 2 of SMART-RNA-Metavirome: a practical RNA metavirome platform compatible with high-throughput sequencing of both short and long reads
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Additional file2: Table S2. Detection of RNA viromes in DENV-infected Ae. albopictus and wild populations of Ae. albopictus using SMART-RNA-Metavirome coupled with Illumina, Oxford Nanopore, and QitanTech Nanopore technologies, as well as a random primer library combined with Illumina Sequencing technology: Virus species identification, RPM, and Proportion of virus detected. a-c) Refer to DENV-infected Ae. albopictus, and wild populations of Ae. albopictus collected from Guangzhou (GZ) and Zhongshan (ZS); d) Random primer library combined with Illumina sequencing; e) SMART-RNA-Metavirome library combined with Illumina sequencing; f) SMART-RNA-Metavirome library combined with Oxford Nanopore sequencing; g) SMART-RNA-Metavirome library combined with QitanTech Nanopore sequencing
附加文件2:表S2。本研究通过SMART-RNA宏病毒组(SMART-RNA-Metavirome)结合Illumina、牛津纳米孔(Oxford Nanopore)及齐碳科技纳米孔(QitanTech Nanopore)测序技术,以及随机引物文库联合Illumina测序技术,对登革病毒(DENV)感染的白纹伊蚊(Aedes albopictus)及其野外种群的RNA病毒组进行检测,分析内容涵盖病毒物种鉴定、每百万映射读数(RPM)及病毒检出比例。a-c) 对应登革病毒感染的白纹伊蚊,以及采集自广州(GZ)与中山(ZS)的白纹伊蚊野外种群;d) 随机引物文库结合Illumina测序;e) SMART-RNA宏病毒组文库结合Illumina测序;f) SMART-RNA宏病毒组文库结合牛津纳米孔测序;g) SMART-RNA宏病毒组文库结合齐碳科技纳米孔测序



