Characterisation of the novel SWR1 complex components.

13 proteins with a positive or unknown NES were identified by MS analysis in at least three of the four co-IP experiments. The initial co-IP was performed with RuvB2 (Tb927.4.2000, the reciprocal co-IPs with the proteins Tb927.10.11690, Tb927.11.5830 and Tb927.7.4040 were performed to confirm the RuvB2 co-IP data). The “Annotation” column indicates the curated annotation that was found for the corresponding accession number in the TriTryp database. The”identified domains” column displays the domains that were found by BLAST search using the NCBI database. The Phyre2 modelling column indicates proteins that were identified by homology modelling. Coverage (Cov.) indicates the coverage in percent between query and template. The confidence (Conf.) represents the relative probability in percent (from 0 to 100) that the match between query and template is a true homology. The nuclear enrichment score (NES; (52)) indicates the probability of a nuclear localisation based on cell fractionation combined with quantitative MS analysis. The column "TrypTag localisation” indicates the cellular localisation of N- and C-terminally tagged proteins. The last column shows in which of the four co-IPs the protein could be identified.